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The culturable bacterial diversity was defined as the number of bacterial species obtained from all media types. The bacterial density was defined as the number of cells observed under a fluorescence microscope and was negatively correlated with the number of isolates. Furthermore, principal component analysis (PCA) revealed the similarity of bacterial species between LGL and LGL-DH as well as between DLS and LGL. The taxonomic affiliation of bacteria from each sample was determined by sequencing the 16S rRNA gene. The taxonomic affiliation of bacterial species in LGL and LGL-DH was similar, which might result from similar nutrient concentrations, and the taxonomic affiliation of bacteria in DLS was mainly related to species from the genera Bacillus and Pseudomonas. Seventeen different alginate producers were isolated from the alga, and alginate-degrading genes (almC and almD) were detected. 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The film was released on May 18, 1921, by Paramount Pictures. Cast Jack Holt as John Driscoll Ruth Roland as Madge Driscoll Charles Kibbe as Red Hennegan Tom Forman as Dick Jamison James Cailloux as Michael O'Brien Elsie Kimball Owen as Pussy Morgan Elaine Linden as Baby References External links Category:1921 films Category:American films Category:English-language films Category:American comedy films Category:1920s comedy films Category:Paramount Pictures films Category:American black-and-white films Category:Films directed by William C. deMille Category:American silent feature filmsLiturgical Music Liturgical Music is a series of three three-hour video tutorials to help people learn how to sing liturgical chants in Latin, English, and other languages. The videos cover both simple and difficult parts of the chant (such as the cantus firmus), demonstrating the appropriate way to breathe, sing, and mensuralize the chant. 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